cryogenic serial block face scanning electron microscopy [FBbi_00100017]
Cryogenic serial block-face scanning electron microscopy is a volumetric imaging technique in which the surface of a biological specimen preserved in its near-native, vitrified state is imaged by a scanning electron microscope, then ablated by a focused ion beam (FIB-SEM) to reveal a new surface, enabling three-dimensional ultrastructural reconstruction of cells and tissues without the artifacts introduced by chemical fixation or resin embedding.
cryogenic serial block face scanning electron microscopy
ID: FBbi_00100017
Class
Cryogenic serial block-face scanning electron microscopy is a volumetric imaging technique in which the surface of a biological specimen preserved in its near-native, vitrified state is imaged by a scanning electron microscope, then ablated by a focused ion beam (FIB-SEM) to reveal a new surface, enabling three-dimensional ultrastructural reconstruction of cells and tissues without the artifacts introduced by chemical fixation or resin embedding.
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Alternative Names
| Synonym | Scope | Reference |
|---|---|---|
| cryo FIB-SEM slice and view | exact synonym | |
| cryo-FIB-SEM serial block face imaging | exact synonym | |
| cryo-serial block face SEM | exact synonym | |
| serial block face cryo-SEM | exact synonym | |
| cryo FIB-SEM subtractive tomography | exact synonym | |
| cryo-serial block face scanning electron microscopy | exact synonym |
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